the bradford assay only works at low protein concentrations

Answers

Answer 1

Answer:

Dilute the sample

Explanation:

Answer 2

The statement is not entirely accurate. The Bradford assay is commonly used to determine protein concentrations in a sample.

However, it is true that the Bradford assay is more accurate and reliable for measuring protein concentrations at low to moderate levels rather than at very high concentrations.

The Bradford assay is based on the binding of Coomassie Brilliant Blue dye to proteins. The dye undergoes a color change from brown to blue when bound to proteins, allowing for the measurement of protein concentrations. At low protein concentrations, the binding of the dye to proteins is well-calibrated and results in a linear relationship between protein concentration and color intensity, making it suitable for accurate quantification.

At high protein concentrations, the binding capacity of the dye may become saturated, leading to non-linear and less accurate measurements. The excess protein can also cause interference and affect color development. In such cases, dilution or alternative protein quantification methods may be more suitable, such as the BCA assay or the Lowry assay.

Therefore, while the Bradford assay can be used to measure protein concentrations across a range of concentrations, it is generally more reliable for low to moderate protein concentrations.

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Related Questions

one difference between a chemical formula and a chemical equation is that the chemical equation shows:

Answers

A chemical reaction taking place

which tool would be best for measuring centimeters
A. Metric ruler
B. Balance scale
C. Meter scale
D. Measuring tape

Answers

Answer:

the answer is d

Explanation:

D. measuring tape
mark brainleist

calculate the energy of a photn of red light with a wavelength of 6.45x10^-5cm

Answers

Answer:

6.45 × 10-5 centimeters

Explanation:

Balance the equation:

Answers

Ca(OH)2 +2 HBr → CaBr2 + 2 H2O 

Which of the following is unlikely to form a compound?

a. S
b. H
c. K
d. Ar
d. Si

Answers

Answer:

I think the answer is Ar, k or just k. Can you pick two answers?

Explanation:

I hope this helps.

P.S. you put two d's

1. How many centimeters are in 13.4 inches?

Answers

34.036 Thats how many

Answer:

34.036

Explanation:

There are 2.54 centimeters in one inch so you multiply 13.4 and 2.54 to get 34.036.

which is the first news paper of Nepal​

Answers

Answer:

Nepal Bhasa Patrika

Explanation:

Nepal Bhasa Patrika

What type of mixture is throughly mixing powdered lemonade with water

Answers

Answer:

I believe it would be a solution I could be wrong tho

Explanation:

Answer:

a solution

Homogeneous mixtures (solutions)

Explanation:

Lemonade is a special type of mixture called a solution.

...................................................................................................................................................

What type of mixture is made by thoroughly mixing powdered lemonade with water?

Homogeneous mixtures (solutions)

Mixtures with substances dissolved evenly into one another, like pink lemonade powder dissolved in water, are called homogeneous mixtures, or solutions.

Match each term in the first column with an example in the second column. On a sheet of paper, write the letter of the answer.

1. physical property
2. chemical property
3. solid.
4. liquid
5. physical change
6. chemical change
———————————————
A. ability to burn
B. rust appears on a nail
C. a puddle freezes
D. substance that has a definite shape and volume
E. temperature or destiny
F. substance that has a definite volume, but no definite shape

Answers

Answer:

4 = f

3 = d

5 = c

6 = b

2 = a

1 = e

Explanation:

How many grams of oxygen would be contained in 4.00 L at 158kPa and 28 C?

Answers

Answer:

it would be 53

Explanation:

because 4.00 L at 158kPa and 28c would be 53 grams of oxygine

001 10.0 points
If you mix 4.5 g of HCl in enough water to
make 0.9 L (900 mL) of solution, what is the
molarity of the solution?
Answer in units of M.

Answers

M=moles of solute/liters solvent

4.5g HCl/36.45 g/mol HCl=0.12 moles

0.12/0.9= 0.14 M

Choose all the answers that apply.

What’s is the freezing point of water?

1. 0’c

2.32’c

3.32’F

4.0’F

Answers

Answer: The answers are the first and third options.

Answer:

1.0'c

3.32'f

Explanation:

What type of chemical reaction is Al + FeO --> Al2O3 + Fe

Answers

Answer:

single reaction equation

Explanation:

Type of Chemical Reaction: For this reaction we have a single replacement reaction. Balancing Strategies: In this single replacement (also called displacement) reaction the Al and Fe are switching places.

Ethanol (C2H5OH) reacts with oxygen gas in a combustion reaction. How many liters of carbon dioxide gas are produced at STP when 375 mL of a 75.0% ethanol solution by mass with a density of 1.22 g/mL reacts with excess oxygen?

Answers

The volume of carbon dioxide produced when 375 mL of a 75.0% ethanol solution by mass with a density of 1.22 g/mL reacts with excess oxygen is  667.52 L.

We are told in the question that the solution contains 75.0% ethanol solution by mass.

Hence;

Density of solution = mass/volume

mass = Density × volume = 1.22 g/mL × 375 mL = 457.5 g

Mass% = mass of solute/ mass of solution × 100

Mass of solute = x

Mass of solution = 457.5 g

Mass percent = 75.0%

Substituting values;

75 = x/457.5 + x × 100

75/100 = x/457.5 + x

0.75(457.5 + x) = x

343.125 + 0.75x = x

343.125  = x -  0.75x

x = 343.125/0.25

x = 1372.5 g

The equation of the reaction is;

C2H6O(l) + 3O2(g) → 2CO2(g) + 3H2O(l)

Number of moles of ethanol =  1372.5 g/46 g/mol = 29.8 moles

From the reaction equation;

2 moles of ethanol produces 2 moles of carbon dioxide

29.8 moles of ethanol also produces 29.8 moles of carbon dioxide

If 1 mole of carbon dioxide occupies 22.4 L

29.8 moles of carbon dioxide occupies 29.8 moles × 22.4 L/ 1 mole

= 667.52 L

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Alison observes that when she injects additional gas molecules into a container of gas, the number of collisions in the container (and the pressure inside) increases. She then increases the volume of the container just enough to restore it to its original pressure. Which of the following laws best describes what Alison observed?
A. Amonton's Law
B. Avogadro's Law
C. Boyles Law
D. Charles's Law
E. Dalton's Law

Answers

Answer:

Explanait it is not d

Compare the structure and bonding of the three compounds:
• carbon dioxide
•magnugum ondo
•silicon dioxide

(6 mark question)

Answers

Silicon dioxide is a covalent network solid. Magnesium oxide is an ionic compound while carbon dioxide is a covalent molecule.

Carbon dioxide is a covalent compound. The compound contains polar C-O bonds but overall the compound is nonpolar as such the molecules are held together by only weak dispersion forces.

The compound magnesium oxide is an ionic solid. It is composed of magnesium ions and oxygen ions in a crystal lattice. Owing to the fact that the ions are very small, the lattice energy is large and the compound is almost insoluble in water.

Silicon dioxide is a covalent network solid which is composed of chains of silicon - oxygen bonds. The network solid forms a very rigid structure having a high melting point.

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Food waste, like a feather or a bone, fall into food, causing
contamination.
Physical
Chemical
Pest
Cross-conta

Answers

Answer:

what do I need to do?..

..

Feather or bone when fall into food causing Physical  contamination.

Food waste, like a feather or a bone etc fall into food causing Physical  contamination because the contaminants such as feather and bone are mixed in the food physically not chemically. These contaminants can be removed through physical means such as with the help of hand.

Some contaminants mix with the food chemically means they react with the food and produce something new. Such type of contaminants are present in liquid form so we can conclude that feather or a bone etc when fall into food causing Physical  contamination.

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Consider the reaction below. 2H2 O2 Right arrow. 2H2O How many moles of water are produced from 13. 35 mol of oxygen? 6. 675 mol 26. 70 mol 53. 40 mol 66. 75 mol.

Answers

Considering the reaction stoichiometry, the correct answer is second option: the total number of moles of water produced from 13. 35 mol of oxygen is 26.7 moles.

The balanced reaction is:

2 H₂ + O₂ → 2 H₂O

By reaction stoichiometry (that is, the relationship between the amount of reagents and products in a chemical reaction), the following amounts of moles of each compound participate in the reaction:

H₂: 2 moles O₂: 1 mole  H₂O: 2 moles

Then you can apply the following rule of three: if by stoichiometry 1 moles of O₂ produce 2 moles of H₂O, 13.35 moles of O₂ will produce how many moles of H₂O?

[tex]amount of moles of H_{2} O=\frac{13.35 moles of O_{2} x2 moles of H_{2} O}{1 mole of O_{2}}[/tex]

amount of moles of H₂O= 26.7 moles

Finally, the correct answer is second option: the total number of moles of water produced from 13. 35 mol of oxygen is 26.7 moles.

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`Carbon Dioxide and Water are the _______ in a chemical reaction. 2. Reactants are also called the starting _______ in a chemical reaction.: Reactants are also called the starting _______ in a chemical reaction. 3. This type of energy comes from the sun: a This type of energy comes from the sun 4. You get this type of energy from food: b You get this type of energy from food 5. The process by which plants use the sun to produce glucose and oxygen.: The process by which plants use the sun to produce glucose and oxygen. 6. The products of photosynthesis: The products of photosynthesis 7. The location of photosynthesis in plant cells: The location of photosynthesis in plant cells 8. Another name for glucose is: Another name for glucose is 9. Energy is provided by this cell organelle during cellular respiration: Energy is provided by this cell organelle during cellular respiration 10. The largest source of energy on earth comes from the: d The largest source of energy on earth comes from the Column B a.Chloroplast b.Ingredients c.Reactants d.Sun e.Radiant energy f.Sugar g.Chemical energy h.Glucose and Oxygen i.Mitochondria j.Photosynthesis

Answers

the answer would be elector literally

Calculate the molar mass of NH4NO3. Molar mass of NH4NO3 = g/mol.

Answers

The molar mass of NH4NO3 in g/mol is 80g/mol.

HOW TO CALCULATE MOLAR MASS:

The molar mass of a compound can be calculated by summing the atomic masses of its constituent elements.

In ammonium nitrate (NH4NO3), there are nitrogen, hydrogen, and oxygen elements.

Atomic mass of nitrogen = 14Atomic mass of oxygen = 16Atomic mass of hydrogen = 1

Molar mass of NH4NO3 = 14 + 1(4) + 14 + 16(3)

Molar mass of NH4NO3 = 80g/mol

Therefore, the molar mass of NH4NO3 in g/mol is 80g/mol.

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Answer:

80g/mol

Explanation:

edge 2022

Rain is-pouring down on you. Your friend asks where so much water
came from. You explain that the water could once have been
a) in a lake.
c) in an ocean.
-b) in-a river.
d) any of the above.

Answers

Answer:

D

Explanation:

It could come from anybody of water around you that had some water evaporated.

The answer is D precipitation comes from all evaporated water

A gas in a rigid container at 25°C has a pressure of 0. 96 atm. A change in temperature causes the pressure to increase to 1. 25 atm. What is the new temperature of the gas? Use StartFraction P subscript 1 over T subscript 1 EndFraction equals StartFraction P subscript 2 over T subscript 2 EndFraction. â€""44. 2°C 32. 6°C 115°C 388°C.

Answers

The new temperature of the gas is 115 °C

From the question given above, the following data were obtained:

Initial temperature (T₁) = 25 °C = 25 + 273 = 298 K Initial pressure (P₁) = 0.96 atmNew pressure (P₂) = 1.25 atmNew temperature (T₂) =?

The new temperature of gas can be obtained as follow:

[tex] \frac{P_1}{T_1} = \frac{P_2}{T_2} \\ \\ \frac{0.96}{298} = \frac{1.25}{T_2} \\ \\ cross \: multiply \\ \\ T_2 \times 0.96 = 298 \times 1.25 \\ \\ divide \: both \: side \: by \: 0.96 \\ \\T_2 = \frac{298 \times 1.25 }{0.96} \\ \\ T_2 = 388 \: K \\ \\ subtract \: 273 \: from \: 388 \: to \: express \: the \: answer \: im \: \degree \: C \\ \\ T_2 = 388 - 273 \\ \\ T_2 = 115 \: \degree \: C [/tex]

Therefore, the new temperature is 115 °C

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4. How are isotopes of the same element different? Select all that apply.
[]They have different masses.
[]They have different numbers of electrons.
[]They have different numbers of neutrons.
[]They have different numbers of protons.

Answers

[tex]\huge \bf༆ Answer ༄[/tex]

The Correct choice is ~ A

Isotopes are the elements with same Atomic Number but different masses ~

For the following hypothetical reaction:

3A + 12B --> 3C

How many moles of B will you need to convert 5 moles of A into as many moles of C as possible?

Answers

Answer:

20

Explanation:

You need 4 times the amount of B, compared to a (there is a 3A:12B which reduces to 1:4), so 4 times 5 is 20.

Hope this helps!

Write word equations for the reactions of a) magnesium with chlorine b) potassium with oxygen c) sodium with bromine d) carbon with oxygen.​

Answers

a) Magnesium + Chlorine -> Magnesium chloride ( Mg + Cl2 -> MgCl2)

b) Potassium + Oxygen -> Potassium oxide
( 2K + O2 -> K2O )

c) Sodium + Bromine -> Sodium bromide
( 2Na + Br2 -> 2NaBr)

d) Carbon + Oxygen -> Carbon dioxide
( C + O2 -> CO2 ) This reaction occurs when heated.

does Waves move in a circular motion.
True or false​

Answers

why yes

Waves are created by energy passing through water, causing it to move in a circular motion

7
How many grams are there in 5 moles of CO2?
A 225.5 g
B) 220.05 g
C)240 g
D) 230 g

Answers

Answer: 44.01g

The molecular mass of carbon dioxide is 44.01amu. The molar mass of any compound is the mass in grams of one mole of that compound. One mole of carbon dioxide molecules has a mass of 44.01g, while one mole of sodium sulfide formula units has a mass of 78.04g. The molar masses are 44.01g/mol and 78.04g/mol respectively
The answer is 220.05

explain how wavelength is related to the energy of a wave

Answers

Answer: as the wavelength gets shorter, the energy increases; as the wavelength gets longer, the energy decreases. The greater the energy, the larger the frequency and the shorter (smaller) the wavelength.

Answer:

Just as wavelength and frequency are related to light, they are also related to energy. The shorter the wavelengths and higher the frequency corresponds with greater energy. So the longer the wavelengths and lower the frequency results in lower energy. The energy equation is E = hν.

Just saying, you have the wrong subject. This should be physics I think.

Chlorine has 7 valence electrons. Describe the type of bond that sodium would have

with chlorine. Write the chemical formula of the compound these two elements would form together. (i figured it out nvm)

Answers

As chlorine has 7 valence electrons and sodium has 1 valence electron so to become stable and complete their octets sodium will become a cation by losing one electron and chlorine will form an anion and will gain one electron in this way both will become stable and an ionic bond will form between sodium and chlorine
Chemical formula:
Na + Cl —> NaCl

discuss why people use the scientific method

Answers

Answer:

They use the scientific method to study different aspects of how people think and behave. They also use the scientific method because it is evidence-based, standardized and objective in conducting experiments.

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